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Get PriceVolume 39 Issue 11 2024 Pages 2024 2024 Pathogens in focus Human papillomavirus E6 and E7 oncogenes Author links open overlay panel Gaëlle Boulet a 1 Caroline Horvath a 1 The link between HPV and cervical cancer led to the development of molecular methods often based on the detection of E6 and E7 for screening and diagnosis
Get PriceCervical cancer is one of the most common cancers in women worldwide and its development is related to two viral oncoproteins E6 and E7 from high risk human papillomaviruses Aberrant
Get PriceFigure 1 The downregulation of HPV16/18 E6/E7 suppresses the proliferative metastatic and glycolytic potential of CC cells A B SiHa and HeLa cells were transfected with HPV16/18 E6/E7 siRNA following which E6/E7 mRNA A and protein B levels were determined by qPCR and western blot respectively C E After HPV16/18 E6/E7 silencing the viability C colony forming ability D and
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Get PriceCervical cancer is intimately associated with infection by certain high risk HPV 3 types such as HPV 16 or HPV 18 At least 90% of cervical carcinomas harbor high risk HPV DNA integrated into the host cell genome and two viral oncogenes E6 and E7 are consistently expressed The high risk HPV E6 and E7 oncoproteins target tumor suppressor signaling pathways that are critical for cellular
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Get PriceThe E6 and E7 genes and LCR of HPV16 virus were sequenced and compared with the HPV16 European prototype reference and other HPV16 mutants for single nucleotide polymorphisms Mu LI Li FX Cui MY Yang Y et al The expression and clinical significance of Sox 11 in cervical carcinogenesis J Xi an Jiaotong Univ Medical Sciences 2024
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Get PriceWe transduced HPV6 11 and 16 E6/E7 genes and EBV LMP 1 gene into primary mouse embryonic fibroblasts The cell lines were examined for indices of transformation activity such as proliferation induction of DNA damage resistance to apoptosis anchorage independent growth and tumor formation in nude mice
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Get PriceThe association of expressions of E cadherin and P cadherin with E6/E7 level in high grade cervical lesions with HPV 16 infection only To examine the expressions of E cadherin and P cadherin and to further explore their relationship with E6/E7 expression 40 cases of cervical lesion tissues with HPV 16 infection only including CINIII n = 23 and cervical cancer n = 17 were collected
Get PriceAuthor Summary Oncogenic human papillomaviruses HPVs are major human carcinogens of broad biomedical importance The growth of HPV positive cervical cancer cells is critically dependent on sustained E6/E7 oncogene expression from endogenous viral DNA sequences We here addressed the question of whether this process is linked to specific E6/E7 dependent alterations of the cellular micro mi
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Get PriceHowever codon optimization of HPV18 E7 and HPV16/18 E6 in pBI 11 and pBI 12 resulted in robust expression of the fusion protein although less than that for pBI 1 Fig 2B Densitometry analysis of the blots further confirmed that HPV16/18 E6 antigen was expressed in larger amounts following vaccination by pBI 11 3 7× and pBI 12 2 8×
Get PriceThe included studies 11 of 3271 publications were of varying methodological quality and predominately performed in a secondary screening setting Sorbye SW et al 2024 HPV E6/E7 mRNA testing is more specific than cytology in post colposcopy follow up of women with negative cervical biopsy PLoS One 6 10 e26022 DOI 10 1371/journal pone
Get PriceThe co expression of HPV 18 E6 and E7 proteins promotes the subcellular redistribution of DLG1 As mentioned before DLG1 deregulation has been observed in histological samples and organotypic raft cultures which expressed not only E6 but also the HPV E7 oncoprotein [16 17 18] Therefore we wanted to investigate in more detail the specific
Get PriceThe HPV mRNA E6/E7 test uses the Aptima HPV mRNA method which is shown to be more specific than an HPV DNA test 1 2 This can lead to fewer false positive results and unnecessary invasive procedures such as colposcopy and biopsy The mRNA test determines the presence of E6/E7 mRNA from 14 high risk HPV genotypes HPV E6/E7 oncoproteins mediate the development of cervical cancer
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